单位文秘网 2021-08-19 09:12:08 点击: 次
摘 要:【目的】探討红树植物在淡水环境中土壤微生态变化,评价红树植物适应性,为红树林在淡水区域引种和推广提供理论基础。【方法】采用Biolog-ECO微平板技术和磷脂脂肪酸标记法(PLFA),探讨淡水驯化条件下红海榄(Rs)、白骨壤(Am)、拉关木(Lr)、秋茄(Kc)、桐花树(Ac)5种不同红树植物根际土壤微生物群落结构特征,揭示红树林根际土壤微生物群落结构对淡水胁迫的响应规律。【结果】研究结果表明:5种红树植物土壤理化性质差异显著;代表微生物整体代谢活性的平均颜色变化率(AWCD)由高到低顺序为Am>Kc>Ac>Rs>Lr,Am土壤微生物代谢活性最强;5种红树植物根际土壤微生物利用碳源类型最多为多聚物;而采用PLFA分析微生物群落结构,5种红树植物共检测到17种PLFA生物标记,16∶0含量最高,Am土壤总PLFA含量显著高于其他4种红树植物;土壤特征微生物分布量最大是细菌;主成分分析表明Lr、Am位于同一主成分,与Biolog研究结果类似。【结论】淡水驯化条件下红树植物土壤质量明显优于无红树林裸滩(CK),5种红树植物根际土壤微生物群落结构存在差异,多聚物是根际土壤微生物的主要利用碳源。表征细菌的生物标记含量最多,在根际土壤微生物中起主导作用的依然是细菌。
关键词:红树林;淡水;土壤微生物;Biolog;PLFA
中图分类号:S 718.5 文献标识码:A文章编号:1008-0384(2019)01-83-12
Abstract: 【Objective】In order to provide a theoretical basis for the introduction and promotion of mangroves in freshwater areas, we explored the micro-ecological changes and adaptability of mangrove plants in freshwater environments.【Method】To explore the microbial community structure characteristics of rhizosphere soil of 5 different mangrove plants, including Rhizophora stylosa(Rs), Avicennia marina(Am), Laguncularia racemose(Lr), Kandelia candel(Kc) and Aegiceras corniculatum(Ac), we performed the study by using Biolog-ECO micro plate (BIOLOG) and phospholipid fatty acid (PLFA) for revealing the response of microbial community structure to freshwater stress.【Result】The results showed that the soil physical and chemical properties were significantly different among 5 mangrove plants. The average well color development (AWCD) values from high to low were as follows: Am >Kc >Ac >Rs >Lr, and value of Am was the highest among 5 treatments. Ploymers was the most utilized carbon source. The PLFAs analysis was performed to explore the changes in the abundance of soil microbes. The results showed that a total of 17 kinds of PLFA were detected in 5 mangrove plants, and the highest content of PLFAs was 16∶0, moreover, the total PLFAs content of Am was significantly higher than the other plants; the group-specific PLFAs in 5 soil samples showed the same trend that content of bacteria reached the maximum. Principal component analysis showed that Lr and Am soils distributed in the same principal component, which was similar to the results of BIOLOG. 【Conclusion】 The results indicated that soil of 5 plants was significantly better than unplanted mangrove soil (CK). The microbial community structure of rhizosphere soil was significantly different among mangrove plants. Soil microorganisms of 5 treatments showed the highest utilization of polymers, moreover, biomarkers of bacteria showed the same trend in 5 treatments.
(责任编辑:单位文秘网) )地址:https://www.kgf8887.com/show-142-85483-1.html
版权声明:
本站由单位文秘网原创策划制作,欢迎订阅或转载,但请注明出处。违者必究。单位文秘网独家运营 版权所有 未经许可不得转载使用